Activities

Our schedule
Intro to DNA || Fruit DNA isolation Video: Pandora's Box ||  || Case study part 1 || Genes in a tube ||  || Case study part 2 || Intro design project ||  || expression Case study part 3 || Principles of gel electrophoresis ||  || Case study part 4 (Role play) || Bioinformatics Synbio video ||  || Work on Projects || PCR amplification of DNA part 1 ||  || Quorum sensing Bonnie Bassler video || PCR amplification of DNA part 2 ||  || Work on projects || Plasmid mini prep ||  || Work on projects || Bacterial transformation part 2 ||  || University Marine Science center ||  ||   || Presentations? ||  || Presentations? || Presentation ||  ||
 * > Day ||> Morning ||> Afternoon ||
 * Monday || Intro to course
 * Monday || Intro to course
 * Tuesday || Intro to Biotech
 * Wednesday || Intro to Synbio
 * Thursday || Control and tuning of gene
 * Friday || Work on projects
 * Saturday ||  ||   ||   ||
 * Sunday ||  ||   ||   ||
 * Monday || Genetic logic gates
 * Tuesday || Two component signaling
 * Wednesday || Bacterial Photography
 * Thursday || open for field trip? || Bacterial transformation part 1 ||  ||
 * Friday || Biosynthetic fuels
 * Saturday ||  ||   ||   ||
 * Sunday ||  ||   ||   ||
 * Monday || Bacterial computing || Human PCR part 1 ||  ||
 * Tuesday || Work on projects || Human PCR part 2 ||  ||
 * Wednesday || Field Trip to Nahant Northeastern
 * Thursday || Work on projects || Work on projects or
 * Friday || Work on projects or
 * Saturday ||  ||   ||   ||

A case study introducing genetic engineering and synthetic biology
Please do not read ahead.

Power Points and videos:
Playing God (history of genetic engineering--YouTube) DNA sequencing--Sanger Method (YouTube) Automated DNA sequencing (YouTube) PCR animation (YouTube) PCR lecture (YouTube) Jay D Keasling: Synthetic Biology Towards Biofuels (YouTube) Chris Voight on engineering cells (YouTube)

Required reading and watching
Synthetic Biology explained (YouTube) [|Decoding Synthetic Biology (YouTube)] [|The Promise and Perils of Synthetic Biology]

The Design Project
BioBuilder Design Assignment

Research topics (some suggestions)
Research a topic and prepare a PowerPoint. If possible try to relate it to your design project. Try searching for these terms with the phrases iGEM and/or synthetic biology Biopharmaceuticals Artemesinin RE coli Man made genome (Venter) Minimal cells Bacterial logic gates Bacterial computing Bacterial counting Artificial chloroplasts Cyanobacterial energy Biosensors and toxins/heavy metals Biosensors and landmines Biosynthetic biofuels Bacterial photography Rewriting the genetic code Mosquito control Quorum sensing Two component signaling system Therapeutics

Our lab experiments
DNA extraction from fruit ( Link to BLASTN)